About RME2015





Monday 20 April 2015
15:45 16:45: Round I

Tuesday 21 April 2015
12:45 13:45: Round II

Wanted: dead or alive!
While real-time PCR (rt-PCR) is a powerful tool for the detection of potentially harmful pathogens, its power is diminished by the inability of the method to discriminate between living and dead, non-harmful cells. Viability rt-PCR uses the DNA masking compound propidium monoazide (PMA), which enters dead cells, intercalates into the DNA, rendering the dead cell DNA undetectable by rt-PCR. Viability PCR thus can be used to increase the power of rt-PCR to detect viable and potentially harmful bacteria in a number of settings of interest to the food industry. In this workshop, QIAGEN will demonstrate the applicability of viability PCR to several areas of interest in the food industry: success of decontamination processes, detection and control of Listeria spp. in dairy manufacture, differentiation of live/dead organisms in poultry husbandry.

Multiplexed test production
SCIENION is a leading supplier of quality instrumentation and services for low volume liquid handling. Proprietary technologies include piezo electric dispensing, surface modifications and microarray manufacturing. Based on SCIENION's core competencies in ultra-low volume liquid dispensing, assay miniaturisation, and microarray manufacturing, we provide our customers with an integrated product portfolio facilitating a number of applications: multiplex assays, DNA microarray production, biosensor loading, miniaturized PCR, peptide microarrays, protein microarrays, cell deposition.

The workshop on multiplexed test production will take a broad view of the current science in diagnostic testing, encompassing new technologies and approaches to diagnostics with a focus on the actual test production. The workshop will cover basic test concepts in molecular diagnostics, assay design, validation, standards and QC, and an update on various platforms and equipment (how to choose the right arrayer in your situation) and SCIENION's view on the future (multiplexing, automation). There will be an opportunity to explore innovative solutions to current test manufacturing, ranging from lateral flow based test-strips to multiplexed and miniaturized ELISA formats. The workshop objective is to discuss the practical implementation of multiplexed tests and the evaluation of field test use of these technologies. Time will be set aside for open discussion, debate and the formation of collaborations.

SCIENION aims to facilitate an environment in which all involved in test manufacturing and diagnostics can learn from each other and share information and experiences. We look forward to welcoming you to this SCIENION workshop on multiplexed test production.

Dispense technology and materials influence on lateral flow test and control lines
BIODOT will demonstrate the new PBT tip and in-line de-gasser used on the XYZ range of movement platforms. This innovation has been produced to further ensure the quality of lines is improved meeting customer's expectations when diagnostic tests are being read in the ever increasing area of quantification and the demanding requirements the reader has on line quality and position. In addition, the contact and none contact Frontline HR and Biojet HR will be demonstrated to show potential differences between the two dispense technologies and their impacts on different nitrocellulose suppliers.



Conference Secretariat: Bastiaanse Communication, P.O. Box 179, NL-3720 AD Bilthoven, the Netherlands

T +31 30 2294247, F +31 30 2252910, RME@bastiaanse-communication.com