WORKSHOPS TUESDAY 26 JANUARY 2010 12.00 - 13.00 Round I 17.00 - 18.30 Round II Workshop 1 AES Chemunex rapid micro testing: flow-cytometry and PCR technology Sponsored and presented by AES Chemunex.

Part I D-Count and Bactiflow technology: rapid microbiology methods for end product control and raw material release for food, cosmetic and non-sterile pharmaceutical products. The D-Count®, BactiFlow®ALS and the BactiFlow® systems, based on flow-cytometry technology, have been specifically designed by AES Chemunex to provide fast microbiological results, from raw material to finished product. All systems are combining speed of detection with high sensitivity level and therefore significantly reduce the time to result for microbiology testing. They can be used to control a wide range of different food, cosmetic and non-sterile pharmaceutical products. This workshop provides a theoretical overview of the labelling and detection technology, automation and available applications. A demonstration of the BactiFlow®ALS system allows to watch the system in operation and to see for yourself the easiness of use. The workshop is also an excellent opportunity to talk to the AES Chemunex product experts and discuss your individual needs.   Part II ADIAFOOD pathogens PCR detection system: an easy approach to food safety! Pathogenic bacteria cause 90% of reported foodborne illnesses. Salmonella, Listeria monocytogenes and E. coli O157H7 have emerged as the most important food pathogens, having a 'zero tolerance' in ready-to eat processed meats and dairy foods. Real time PCR technology allows laboratories to quickly and accurately detect pathogens in raw ingredients, finished products and environmental samples. But identifying low levels of these pathogens in food is a challenge, so industry is required to combine better enrichment broths and accurate detection systems. Thanks to its experience in food microbiology, AES Chemunex developed the Adiafood® range to answer this need. Adiafood® is a complete detection solution including an enrichment broth and a real time PCR kit using 2 specificity levels (specific primers and probes) for an accurate identification of different pathogens. After 18 h of enrichment, Adiafood® PCR system performs a PCR thermocycling process with results available in 3 h: it results in an overall TTR of 21 h for pathogens detection. This technology has been designed to simplify PCR analysis as it utilises a single amplification protocol for all the pathogens and does not need any curves interpretation. This workshop provides a theoretical overview of the three analysis steps and the advantage of a specific and rapid PCR detection system. It is also the opportunity to organize an easy demonstration of Adiafood® system with a multipathogenic assay in the same run. Moreover the workshop is an excellent opportunity to talk to the AES Chemunex product experts and discuss your individual needs.

Workshop 2 Rapid tests for allergens and mycotoxins Sponsored and presented by R-Biopharm AG. R-Biopharm offers a wide range of analytical test kits for food and feed analysis, and clinical diagnostics.

Part I New developments in rapid mycotoxin detection Introduction of technical background of lateral flow based test systems Reading and documentation of results by RIDA QUICK SCAN Hands-on training of the RIDA QUICK DON detection method Discussion with the experts   Part II Rapid lateral flow tests in a HACCP based approach for allergen monitoring Introduction of technical background Presentation of the product range Hands-on training of the method Discussion with the experts

Workshop 3 Portable and handheld systems for rapid molecular and immunodiagnostics Sponsored and presented by ESE GmbH. Molecular Diagnostics for DNA/RNA testing has become rapid since the invention of TwistDX's revolutionary isothermal nucleic acid amplification technology called RPA and the highly sensitive fluorescence readers called ESE-Quant provided by ESE GmbH. We will present an overview of the technologies involved and show examples for both molecular and immunodiagnostics testing in less than 10 minutes starting from crude samples to the fully interpreted result. The stand-alone devices demonstrate operational and physical robustness; they can be operated by layman and can be produced affordably.

Part I Introduction and short presentation   Part II Practical session and demonstration

Workshop 4 Development of rapid assays for the veterinarian, agricultural and human diagnostic fields Presented by Wageningen University and Research Centre. As an international knowledge-based organisation Wageningen University and Research Centre (Wageningen UR) carries out research throughout the knowledge chain in the fields of life sciences and natural resources. Research comprises fundamental as well as applied research. Within the different specialized research institutes of Wageningen UR a broad variety of technologies are used to develop and validate assays for clients. In addition, the development of customer-defined applications as well as the development of new innovative technologies are also part of the working area. Many diagnostic tools have already been developed in close collaboration with industrial partners. Strategic investments ensure the maintenance of strong intellectual property (IP) positions in the veterinarian, agricultural and human diagnostic fields. In this workshop a brief introduction of some innovative technologies currently under study, including some practical demonstrations, will be given.

Part I Technologies and their possibilities Wageningen UR is developing assays for, e.g., biosensor technology, xMAP technology, microfluidic interferometric device, lateral flow, DNA and protein microarrays   Part II Practical demonstration Several technologies will be demonstrated.

Workshop 5 Quantitative POC tests - research to manufacture - tools and approaches to speed your development and commercialisation of rapid tests Sponsored and presented by BioDot Ltd. BioDot enables, inspires and educates scientists to commercialise their ideas from R&D through to manufactured product. Using its core competencies in low volume dispensing, cutting and laminating BioDot has developed a range of equipment for the research, development and manufacture of diagnostic tests.

Part I Demonstration: dispensing, contact, non-contact, lamination of materials, cutting of sticks, and assembly   Part II Equipment on display and in operation New BioDot materials handling equipment, including dispensing, lamination, cutting, and assembly

 

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